Application
Immunohistochemistry of CDX2 in rat brain tissue with CDX2 antibody at 5 μg/mL.Application
Immunofluorescence of CDX2 in rat brain tissue with CDX2 antibody at 20 μg/mL.Application
Western blot analysis of CDX2 in mouse brain tissue lysate with CDX2 antibody at 1 μg/mL.| Product Name | CDX2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDX2 Antibody: CDX3, CDX-3, CDX3, Homeobox protein CDX-2 |
| Product description | CDX2 Antibody: CDX2 is a member of the caudal homeobox family of transcription factors that also includes the proteins CDX1 and CDX4. CDX2 is strongly expressed in the trophectoderm in the blastocyst with expression maintained within the proliferating extra-embryonic ectoderm during development. It is required for the repression of the transcription of the POU5F1/Oct4 and NANOG genes in the outer cells of the blastocyst and is thus essential for the segregation of the inner cell mass and trophectoderm at the blastocyst stage. Aberrant expression of CDX2 is associated with numerous types of carcinomas, including intestinal metaplasia, gastric carcinoma, and acute myeloid leukemia due to deregulated HOX gene expression.1) Beck F, Erler T, Russell A, et al. Expression of CDX-2 in the mouse embryo and placenta: possible role in patterning of the extra-embryonic membranes. Dev. Dyn.1995; 204:219-27. |
| Immunogen | CDX2 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human CDX2. The immunogen is located within amino acids 220 - 270 of CDX2. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| CDX2 antibody can be used for detection of CDX2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDX2 |
|---|---|
| Alternative Names | CDX2 Antibody: CDX3 CDX-3 CDX3 Homeobox protein CDX-2 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Chicken: (94%) |
Application
Immunohistochemistry of CDX2 in rat brain tissue with CDX2 antibody at 5 μg/mL.
Application
Immunofluorescence of CDX2 in rat brain tissue with CDX2 antibody at 20 μg/mL.
Application
Western blot analysis of CDX2 in mouse brain tissue lysate with CDX2 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1403 - Mouse Brain Tissue Lysate |
|---|---|
| Application Notes | CDX2 antibody can be used for detection of CDX2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CDX2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CDX2 Antibody is supplied in PBS containing 0.02% sodium azide. |
