Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Product Name | CEBPA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CEBPA, CEBP, C/EBP-alpha |
| Product description | CEBPA is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. It has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, CEBPA can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells.1) Zhu, Q.S., et al., (2004) J. Biol. Chem. 279 (29), 29902-29910. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CEBPA. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| CEBPA antibody can be used for detection of CEBPA by ELISA at 1:312500. CEBPA antibody can be used for detection of CEBPA by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CEBPA |
|---|---|
| Alternative Names | CEBPA CEBP C/EBP-alpha |
| Molecular Weight(MW) | 38 kDa |
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | CEBPA antibody can be used for detection of CEBPA by ELISA at 1:312500. CEBPA antibody can be used for detection of CEBPA by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store CEBPA antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
