Application
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of CEBPA Antibody in mouse liver tissue lysates (35ug/lane)| Product Name | CEBPA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CCAAT/enhancer-binding protein alpha, C/EBP alpha, CEBPA |
| Product description | CEBPA is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. This protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, the protein can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells.1) Twu, Y.C., et al. Blood 115(12):2491-2499(2010) |
| Immunogen | This CEBPA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-330 amino acids from the C-terminal region of human CEBPA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CEBPA |
|---|---|
| Alternative Names | CCAAT/enhancer-binding protein alpha C/EBP alpha CEBPA |
| Molecular Weight(MW) | 38 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of CEBPA Antibody in mouse liver tissue lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
