Application
Immunofluorescence of CEBPD in human spleen tissue with CEBPD antibody at 20 μg/mL.Application
Western blot analysis of CEBPD in rat spleen tissue lysate with CEBPD antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | CEBPD Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CEBPD Antibody: CELF, CRP3, C/EBP-delta, NF-IL6-betaNuclear factor NF-IL6-beta, NF-IL6-beta |
| Product description | CEBPD Antibody: CCAAT enhancer binding proteins (CEBPs) are a family of transcription factors that all contain a highly conserved, basic-leucine zipper domain at the C-terminus that is involved in dimerization and DNA binding. At least six members of the family have been isolated and characterized to date (CEBP alpha to CEBP zeta). CEBPD is a leucine zipper (LZ) DNA-binding protein that regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. CEBPD is an important transcriptional activator in the regulation of genes involved in immune and inflammatory responses and has been reported to possess many tumor suppressor-like properties.1) Williams SC, Cantwell CA, Johnson PF, et al. A family of C/EBP-related proteins capable of forming covalently linked leucine zipper dimers in vitro. Genes Dev.1991; 5:1553-67. |
| Immunogen | Rabbit polyclonal CEBPD antibody was raised against an 18 amino acid peptide near the carboxy terminus of human CEBPD. The immunogen is located within amino acids 170 - 220 of CEBPD. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| CEBPD antibody can be used for detection of CEBPD by Western blot at 0.5 - 1 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CEBPD |
|---|---|
| Alternative Names | CEBPD Antibody: CELF CRP3 C/EBP-delta NF-IL6-betaNuclear factor NF-IL6-beta NF-IL6-beta |
| Molecular Weight(MW) | 30 kDa |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
| Tissue Specificity | Multiple transcript variants encoding different isoforms have been found for this gene. |
Application
Immunofluorescence of CEBPD in human spleen tissue with CEBPD antibody at 20 μg/mL.
Application
Western blot analysis of CEBPD in rat spleen tissue lysate with CEBPD antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1466 - Rat Spleen Tissue Lysate |
|---|---|
| Application Notes | CEBPD antibody can be used for detection of CEBPD by Western blot at 0.5 - 1 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CEBPD antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CEBPD Antibody is supplied in PBS containing 0.02% sodium azide. |
