Application
Western blot analysis of anti-CSE1L Pab in, from left to right, A375, CEM, and mouse heart cell line lysates (35ug/lane).Application
Western blot analysis of lysates from Hela, Jurkat, Ramos, mouse NIH/3T3 cell line (from left to right), using CSE1L Antibody at 1:1000 at each lane.Application
Immunohistochemical analysis of paraffin-embedded H. tonsil section using CSE1L Antibody. Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.Application
Fluorescent image of HeLa cells stained with Cellular Apoptosis Susceptibility Antibody . AP1935a was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).Application
Immunohistochemical analysis of paraffin-embedded H. stomach section using CSE1L Antibody. Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.| Product Name | Cellular Apoptosis Susceptibility Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Proteins that carry a nuclear localization signal (NLS) are transported into the nucleus by the importin-alpha/beta heterodimer. Importin-alpha binds the NLS, while importin-beta mediates translocation through the nuclear pore complex. After translocation, RanGTP binds importin-beta and displaces importin-alpha. Importin-alpha must then be returned to the cytoplasm, leaving the NLS protein behind. CSE1L binds strongly to NLS-free importin-alpha, and this binding is released in the cytoplasm by the combined action of RANBP1 and RANGAP1. In addition, CSE1L may play a role both in apoptosis and in cell proliferation.1) Goldberg, G.S., et al., J. Biol. Chem. 278(47):46533-46540 (2003). |
| Immunogen | This Cellular Apoptosis Susceptibility antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-84 amino acids from the N-terminal region of human Cellular Apoptosis Susceptibility. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For IHC-P starting dilution is: 1:100 For IF starting dilution is: 1:100 For WB starting dilution is: 1:1000 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | CSE1L |
|---|---|
| Alternative Names | Exportin-2 Exp2 Cellular apoptosis susceptibility protein Chromosome segregation 1-like protein Importin-alpha re-exporter CSE1L CAS XPO2 |
| Molecular Weight(MW) | 110 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Zebrafish |
Application
Western blot analysis of anti-CSE1L Pab in, from left to right, A375, CEM, and mouse heart cell line lysates (35ug/lane).
Application
Western blot analysis of lysates from Hela, Jurkat, Ramos, mouse NIH/3T3 cell line (from left to right), using CSE1L Antibody at 1:1000 at each lane.
Application
Immunohistochemical analysis of paraffin-embedded H. tonsil section using CSE1L Antibody. Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Application
Fluorescent image of HeLa cells stained with Cellular Apoptosis Susceptibility Antibody . AP1935a was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Application
Immunohistochemical analysis of paraffin-embedded H. stomach section using CSE1L Antibody. Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.| Application Notes | For IHC-P starting dilution is: 1:100 For IF starting dilution is: 1:100 For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
