Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Antibody is used in Western blot to detect cGKI beta in 293 cell lysate (Lane 1) and mouse small intestine tissue lysate (Lane 2).| Product Name | cGKI Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | cGMP-dependent protein kinase 1, cGK 1, cGK1, cGMP-dependent protein kinase I, cGKI, PRKG1, PRKG1B, PRKGR1A, PRKGR1B |
| Product description | Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.1) Orstavik, S., et al., Genomics 42(2):311-318 (1997). |
| Immunogen | This cGKI (cGKI beta) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 629-660 amino acids from the C-terminal region of human cGKI beta. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | PRKG1 |
|---|---|
| Alternative Names | cGMP-dependent protein kinase 1 cGK 1 cGK1 cGMP-dependent protein kinase I cGKI PRKG1 PRKG1B PRKGR1A PRKGR1B |
| Molecular Weight(MW) | 76 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Antibody is used in Western blot to detect cGKI beta in 293 cell lysate (Lane 1) and mouse small intestine tissue lysate (Lane 2).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
