Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.Application
Antibody used in IHC on Human kidney.| Product Name | CHEK1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CHEK1, CHK1 |
| Product description | CHEK1 is required during normal S phase to avoid aberrantly increased initiation of DNA replication, thereby protecting against DNA breakage. Its expression is dispensable for somatic cell death and critical for sustaining G2 DNA damage checkpoint.1) Kramer, A., et al., (2004) Nat. Cell Biol. 6 (9), 884-891. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CHEK1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| CHEK1 antibody can be used for detection of CHEK1 by ELISA at 1:312500. CHEK1 antibody can be used for detection of CHEK1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CHEK1 |
|---|---|
| Alternative Names | CHEK1 CHK1 |
| Molecular Weight(MW) | 54 kDa |
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Application
Antibody used in IHC on Human kidney.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | CHEK1 antibody can be used for detection of CHEK1 by ELISA at 1:312500. CHEK1 antibody can be used for detection of CHEK1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store CHEK1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
