Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Chk1 (Ab-317).Application
Western blot analysis of lysed extracts from MDA231, HeLa and 293 cells using Chk1 (Ab-317).| Product Name | Chk1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CHK1, PKCB, PRKCB1, CHEK1 |
| Product description | Chek1 is a protein kinase that inhibits mitotic entry after DNA damage, required for the DNA damage checkpoint and is strongly similar to murine Chek1. Checkpoint pathways control the order and timing of cell cycle transitions and ensure that critical events, such as DNA replication and chromosome segregation, are completed with high fidelity. The mouse and human proteins share 90% sequence identity through the protein kinase domains. The sequence of the 476-amino acid human Chek1 protein is 29%, 40%, and 44% identical to those of the fission yeast Chek1, C. elegans Chek1, and Drosophila 'grapes' (Grp) proteins, respectively. Chek1 is expressed ubiquitously as an approximately 2.4-kb mRNA, with the most abundant expression in thymus, testis, small intestine, and colon. The protein has altered mobility when isolated from cells treated with ionizing radiation, indicating that Chek1 is modified in response to DNA damage. In vitro, Chek1 directly phosphorylates a regulator of CDC2 tyrosine phosphorylation, CDC25C. In response to DNA damage, Chek1 phosphorylates and inhibits CDC25C, thus preventing activation of the CDC2-Cyclin-B complex and mitotic entry1) Zhang YW, et al. (2005) Mol Cell; 19 (5): 607-18. Bhoumik A, et al. (2005) Mol Cell; 18 (5): 577-87. Rocha S, et al. (2005) EMBO J. Clarke CA, et al. (2005) Biochem J. Yu X, et al. (2004) Mol Cell Biol; 24 (21): 9478-86. |
| Immunogen | Chk1 (Ab-317) antibody was raised against a peptide sequence around aa.315~319 (S-S-S-Q-P) derived from Human Chk1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
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| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CHEK1 |
|---|---|
| Alternative Names | CHK1 PKCB PRKCB1 CHEK1 |
| Molecular Weight(MW) | 56 kDa |
| Tissue Specificity | This antibody detects endogenous level of total Chk1 protein. |
| Entrez Gene | 1111 |
|---|---|
| Protein Accession | NP_001107593.1 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Chk1 (Ab-317).
Application
Western blot analysis of lysed extracts from MDA231, HeLa and 293 cells using Chk1 (Ab-317).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
