Application
Western blot analysis in Hela cell line and mouse thymus tissue lysates (35ug/lane).| Product Name | CHK1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | HRD1 is a ubiquitin ligase whose expression is induced by the unfolded protein response (UPR) following endoplasmic reticulum stress. Expression of HRD1 protects cells from apoptosis by inducing degradation of abnormally processed proteins that accumulate in the endoplasmic reticulum. HRD1 is expressed in many tissues, strongly expressed in brain, pancreas, liver, kidney and skeletal muscle. Amano T, et al. reported that Synoviolin/Hrd1 (expressed in rheumatoid synovium) is a novel causative factor for arthropathy by triggering synovial cell outgrowth through its antiapoptotic effects. HRD1 contains one ring-type zinc finger.1) Kaneko M, FEBS Lett. 2002. 532: 147-152. |
| Immunogen | This CHK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 258-287 amino acids from human CHK1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CHEK1 |
|---|---|
| Alternative Names | Serine/threonine-protein kinase Chk1 CHK1 checkpoint homolog Cell cycle checkpoint kinase Checkpoint kinase-1 CHEK1 CHK1 |
| Molecular Weight(MW) | 54 kDa |
Application
Western blot analysis in Hela cell line and mouse thymus tissue lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
