Application
Western blot analysis of extract from HL60 cells using Chk2 (Ab-516).| Product Name | Chk2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDS1, CHK2, LFS2, RAD53, hCds1, HuCds1, PP1425, NFKB3, CHEK2 |
| Product description | The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Plays a central role in DNA repair by facilitating cellular response to DNA repair. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation1) Melchionna, R. et al. (2000) Nat. Cell Biol. 2, 762-765. Ahn, J. Y. et al. (2000) Cancer Res. 60, 5934-5936. Lee, C. H. and Chung, J. H. (2001) J. Biol. Chem. 276, 30537-30541 |
| Immunogen | Chk2 (Ab-516) antibody was raised against a peptide sequence around aa. 514~518 (Q-P-S-T-S) derived from Chk2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CHEK2 |
|---|---|
| Alternative Names | CDS1 CHK2 LFS2 RAD53 hCds1 HuCds1 PP1425 NFKB3 CHEK2 |
| Molecular Weight(MW) | 62 kDa |
| Tissue Specificity | This antibody detects endogenous levels of total Chk2 protein. |
| Entrez Gene | 11200 |
|---|---|
| Protein Accession | NP_001005735.1 |
Application
Western blot analysis of extract from HL60 cells using Chk2 (Ab-516).| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
