Application
Immunocytochemistry of Chondroadherin in 3T3 cells with Chondroadherin antibody at 5 μg/ml.Application
Western blot analysis of Chondroadherin in 3T3 cell lysate with Chondroadherin antibody at 1 μg/ml.| Product Name | Chondroadherin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cartilage leucine-rich protein, CHAD, SLRR4A |
| Product description | Chondroadherin, also known as CHAD or SLRR4A, belongs to the small leucine-rich proteoglycan (SLRP) family and class IV subfamily and plays a significant role in the regulation of chondrocyte growth and proliferation (1,2). It localizes to the extracellular matrix and contains nine tandem leucine repeats as well as N-terminal and C-terminal leucine rich domains (2-4). Chondroadherin is implicated in the adhesion of fibroblasts, osteoblasts and chondrocytes, which is mediated by interactions with Integrin alpha2 beta1 (2,4,5).1) Neame PJ, Sommarin Y, Boynton RE, et al. The structure of a 38-kDa leucine-rich protein (chondroadherin) isolated from bovine cartilage. J. Biol. Chem. 1994; 269:21547-54. |
| Immunogen | Chondroadherin antibody was raised against a 17 amino acid peptide near the carboxy terminus of human Chondroadherin. The immunogen is located within the last 50 amino acids of Chondroadherin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| Chondroadherin antibody can be used for detection of Chondroadherin by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CHAD |
|---|---|
| Alternative Names | Cartilage leucine-rich protein CHAD SLRR4A |
| Molecular Weight(MW) | Predicted: 38 kDa Observed: 36 kDa |
| Sequence Similarities | 2) Cat. No. 17-201 - 3T3/BALB Cell Slide |
| Tissue Specificity | Chondroadherin antibody is human and mouse reactive. Chondroadherin antibody is predicted to not cross-react with CHADL protein. |
Application
Immunocytochemistry of Chondroadherin in 3T3 cells with Chondroadherin antibody at 5 μg/ml.
Application
Western blot analysis of Chondroadherin in 3T3 cell lysate with Chondroadherin antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1210 - 293 Cell Lysate |
|---|---|
| Application Notes | Chondroadherin antibody can be used for detection of Chondroadherin by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Chondroadherin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | Chondroadherin antibody is supplied in PBS containing 0.02% sodium azide. |
