Nicotinic acetylcholine receptors (nAChRs), such as CHRNA5, are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be (hetero)pentamers composed of homologous subunits.1) Groot-Kormelink, P.J., et al., (2001) Br. J. Pharmacol. 134 (4), 789-796.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CHRNA5.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
CHRNA5 antibody can be used for detection of CHRNA5 by ELISA at 1:12500. CHRNA5 antibody can be used for detection of CHRNA5 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
CHRNA5 antibody can be used for detection of CHRNA5 by ELISA at 1:12500. CHRNA5 antibody can be used for detection of CHRNA5 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store CHRNA5 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt