Application
Western blot analysis of lysate from Daudi cell line, using CHRNA9 Antibody at 1:1000.Application
Flow cytometric analysis of Jurkat cells using CHRNA9 Antibody (N-term)(green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.| Product Name | CHRNA9 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Neuronal acetylcholine receptor subunit alpha-9, Nicotinic acetylcholine receptor subunit alpha-9, NACHR alpha-9, CHRNA9, NACHRA9 |
| Product description | Ionotropic receptor with a probable role in the modulation of auditory stimuli. Agonist binding may induce an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is permeable to a range of divalent cations including calcium, the influx of which may activate a potassium current which hyperpolarizes the cell membrane. In the ear, this may lead to a reduction in basilar membrane motion, altering the activity of auditory nerve fibers and reducing the range of dynamic hearing. This may protect against acoustic trauma. May also regulate keratinocyte adhesion.1) Sgard F.,et al.Mol. Pharmacol. 61:150-159(2002). |
| Immunogen | This CHRNA9 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 8-42 amino acids from the N-terminal region of human CHRNA9. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For FACS starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CHRNA9 |
|---|---|
| Alternative Names | Neuronal acetylcholine receptor subunit alpha-9 Nicotinic acetylcholine receptor subunit alpha-9 NACHR alpha-9 CHRNA9 NACHRA9 |
| Molecular Weight(MW) | 55 kDa |
Application
Western blot analysis of lysate from Daudi cell line, using CHRNA9 Antibody at 1:1000.
Application
Flow cytometric analysis of Jurkat cells using CHRNA9 Antibody (N-term)(green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.| Application Notes | For FACS starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
