Application
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in HL-60 cell line lysates (35ug/lane).| Product Name | CHST2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Carbohydrate sulfotransferase 2, 282-, Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 2, GST-2, N-acetylglucosamine 6-O-sulfotransferase 1, GlcNAc6ST-1, Gn6ST-1, CHST2, GN6ST |
| Product description | N-acetylglucosamine-6-O-sulfotransferases, such as CHST2, catalyze the transfer of sulfate from 3-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) to position 6 of a nonreducing N-acetylglucosamine (GlcNAc) residue (Uchimura et al., 1998 [PubMed 9722682]).1) Shimada, M., et al. Hum. Genet. 128(4):433-441(2010) |
| Immunogen | This CHST2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 309-335 amino acids from the Central region of human CHST2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CHST2 |
|---|---|
| Alternative Names | Carbohydrate sulfotransferase 2 282- Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 2 GST-2 N-acetylglucosamine 6-O-sulfotransferase 1 GlcNAc6ST-1 Gn6ST-1 CHST2 GN6ST |
| Molecular Weight(MW) | 58 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in HL-60 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
