Application
Immunofluorescence of CIKS in Human Lung cells with CIKS antibody at 20 μg/mL.Application
Immunohistochemistry of CIKS in human lung tissue with CIKS antibody at 5 μg/mL.Application
Western blot analysis of CIKS expression in human placenta tissue lysate with CIKS antibody at 1 μg /ml.| Product Name | CIKS Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CIKS Antibody: ACT1, CIKS, C6orf2, C6orf4, C6orf5, C6orf6, CANDF8, PSORS13, Adapter protein CIKS, Nuclear factor NF-kappa-B activator 1, ACT1 |
| Product description | CIKS Antibody: Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-κB associates with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. IκB is phosphorylated by IκB kinase (IKK) complex that contains IKKα, IKKβ, and IKKγ. A novel molecule that associates with and activates IKK was recently identified and designated CIKS (for connection to IKK and SAPK/JNK) and Act1 (for NF-κB activator 1). CIKS directly interacts with IKKγ. CIKS/Act1 also activates activating transcription factor (ATF) and activator protein 1 (AP-1) through Jun kinase (JNK). These results indicate that CIKS/Act1 is involved in the inflammation and stress responses. CIKS/Act1 is ubiquitously expressed in human tissues.1) Leonardi A, Chariot A, Claudio E, Cunningham K, Siebenlist U. CIKS, a connection to Iκ B kinase and stress-activated protein kinase. Proc Natl Acad Sci USA. 2000;97(19):10494-9. |
| Immunogen | CIKS antibody was raised against a 15 amino acid peptide near the carboxy terminus of human CIKS. The immunogen is located within the last 50 amino acids of CIKS. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| CIKS antibody can be used for detection of CIKS by Western blot at 1 μg/mL. A band at approximately 63 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TRAF3IP2 |
|---|---|
| Alternative Names | CIKS Antibody: ACT1 CIKS C6orf2 C6orf4 C6orf5 C6orf6 CANDF8 PSORS13 Adapter protein CIKS Nuclear factor NF-kappa-B activator 1 ACT1 |
| Molecular Weight(MW) | 63 kDa |
| Sequence Similarities | 3) Cat. No. 10-101 - Human Lung Tissue Slide |
Application
Immunofluorescence of CIKS in Human Lung cells with CIKS antibody at 20 μg/mL.
Application
Immunohistochemistry of CIKS in human lung tissue with CIKS antibody at 5 μg/mL.
Application
Western blot analysis of CIKS expression in human placenta tissue lysate with CIKS antibody at 1 μg /ml.| Positive Control | 1) Cat. No. 1309 - Human Placenta Tissue Lysate |
|---|---|
| Application Notes | CIKS antibody can be used for detection of CIKS by Western blot at 1 μg/mL. A band at approximately 63 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CIKS antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CIKS Antibody is supplied in PBS containing 0.02% sodium azide. |
