Application
Immunohistochemistry of CIP75 in mouse kidney tissue with CIP75 antibody at 5 μg/mL.Application
Western blot analysis of CIP75 in 3T3 cell lysate with CIP75 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | CIP75 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CIP75 Antibody: A1U, A1Up, UBIN, CIP75, C1orf6, Ubiquilin-4, Ataxin-1 interacting ubiquitin-like protein |
| Product description | CIP75 Antibody: The ubiquitin-proteosome pathway of protein degradation is one of the mechanisms that ensure the proper level of cellular proteins. The ubiquitin-like (UbL) and ubiquitin-associated (UBA) domain containing protein family is thought to be involved in proteosomal degradation. One such protein is CIP75, also known as ubiquilin-4, interacts with a number of proteins such as Ataxin-1 and Connexin-43, resulting in an increased rate of turnover of these proteins. Overexpression CIP75 led to a significant reduction of Connexin-43 half-life, with the opposite being observed in siRNA knockdown experiments. CIP75 contains an N-terminal UbL domain which is thought to interact with proteins of the 26S proteosome complex and a C-terminal UBA domain which appears to mediate its interaction with Connexin-75. At least three isoforms of CIP75 are known to exist.1) Su V and Lau AF. Ubiquitin-like and ubiquitin-associated domain proteins: significance in proteasomal degradation. Cell. Mol. Life Sci.2009; 66:2819-33. |
| Immunogen | CIP75 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human CIP75. The immunogen is located within the first 50 amino acids of CIP75. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| CIP75 antibody can be used for detection of CIP75 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UBQLN4 |
|---|---|
| Alternative Names | CIP75 Antibody: A1U A1Up UBIN CIP75 C1orf6 Ubiquilin-4 Ataxin-1 interacting ubiquitin-like protein |
Application
Immunohistochemistry of CIP75 in mouse kidney tissue with CIP75 antibody at 5 μg/mL.
Application
Western blot analysis of CIP75 in 3T3 cell lysate with CIP75 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate |
|---|---|
| Application Notes | CIP75 antibody can be used for detection of CIP75 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CIP75 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CIP75 Antibody is supplied in PBS containing 0.02% sodium azide. |
