Application
Western blot analysis of CITED2 in Jurkat cell lysate with CITED2 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of CITED2 in Jurkat cells with CITED2 antibody at 20 μg/mL.| Product Name | CITED2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CITED2 Antibody: ASD8, MRG1, VSD2, MRG-1, P35SRJ, MSG-related protein 1 |
| Product description | CITED2 Antibody: CITED2 was initially identified as a potential transcriptional activator with significant homology to MSG1, a melanocyte-specific nuclear protein. CITED2 interacts with the histone acetyltransferase p300/CBP and acts as a coactivator to several DNA-binding transcription factors such as HIF-1 and AP-2. CITED2 also controls proliferation of mouse embryonic fibroblasts via the polycomb group genes Bmi-1 and Mel18 and the tumor suppressor Ink4a/Arf. CITED2 has also been found to be an essential regulator of adult hematopoietic stem cells (HSCs), with conditional deletion of CITED2 in the adult mouse resulting in the loss of HSCs, multilineage bone marrow failure and increased lethality.1) Shioda T, Fenner MH, and Isselbacher KJ. MSG1 and its related protein MRG1 share a transcription activating domain. Gene 1997; 204:235-41. |
| Immunogen | CITED2 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human CITED2. The immunogen is located within the first 50 amino acids of CITED2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| CITED2 antibody can be used for detection of CITED2 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CITED2 |
|---|---|
| Alternative Names | CITED2 Antibody: ASD8 MRG1 VSD2 MRG-1 P35SRJ MSG-related protein 1 |
| Molecular Weight(MW) | 30 kDa |
| Sequence Similarities | 2) Cat. No. 17-005 - Jurkat Cell Slide |
| Tissue Specificity | Multiple isoforms of CITED2 are known to exist. |
| Entrez Gene | 10370 |
|---|---|
| Protein Accession | NP_001161861 |
Application
Western blot analysis of CITED2 in Jurkat cell lysate with CITED2 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of CITED2 in Jurkat cells with CITED2 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | CITED2 antibody can be used for detection of CITED2 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CITED2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CITED2 Antibody is supplied in PBS containing 0.02% sodium azide. |
