Application
Immunofluorescence of CLAUDIN4 in human colon tissue with CLAUDIN4 antibody at 20 μg/mL.Application
Western blot analysis of CLAUDIN4 in HeLa cell lysate with CLAUDIN4 antibody at 0.5 μg/mL.| Product Name | CLAUDIN4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CLAUDIN4 Antibody: CPER, CPE-R, CPETR, CPETR1, WBSCR8, hCPE-R, CPER, Claudin-4, Clostridium perfringens enterotoxin receptor |
| Product description | CLAUDIN4 Antibody: CLAUDIN4 is an integral membrane protein belonging to the claudin family, a family of cellular adhesion molecules that are components of tight junctions. CLAUDIN4 is a component of tight junction strands and may play a role in internal organ development and function during pre- and postnatal life. The CLAUDIN4 gene is deleted in Williams-Beuren syndrome, a neurodevelopmental disorder affecting multiple systems. CLAUDIN4 is frequently overexpressed in ovarian and other epithelial cancers.1) Mitic LL, Unger VM, and Anderson JM. Expression, solubilization, and biochemical characterization of the tight junction transmembrane protein claudin-4. Protein Sci. 2003; 12:218-27. |
| Immunogen | CLAUDIN4 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human CLAUDIN4. The immunogen is located within the last 50 amino acids of CLAUDIN4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| CLAUDIN4 antibody can be used for detection of CLAUDIN4 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CLDN4 |
|---|---|
| Alternative Names | CLAUDIN4 Antibody: CPER CPE-R CPETR CPETR1 WBSCR8 hCPE-R CPER Claudin-4 Clostridium perfringens enterotoxin receptor |
| Molecular Weight(MW) | Predicted: 23 kDa Observed: 23 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (94%) |
| Tissue Specificity | At least four isoforms of CLAUDIN4 are known to exist; CLAUDIN4 antibody will detect all four isoforms |
Application
Immunofluorescence of CLAUDIN4 in human colon tissue with CLAUDIN4 antibody at 20 μg/mL.
Application
Western blot analysis of CLAUDIN4 in HeLa cell lysate with CLAUDIN4 antibody at 0.5 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | CLAUDIN4 antibody can be used for detection of CLAUDIN4 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CLAUDIN4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CLAUDIN4 Antibody is supplied in PBS containing 0.02% sodium azide. |
