Application
Antibody used in WB on Human kidney at 0.2-1 ug/ml.| Product Name | CLCNKB Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CLCNKB, CLCKB, ClC-K2, ClC-Kb |
| Product description | Chloride channel Kb (CLCNKB) is a member of the CLC family of voltage-gated chloride channels, which comprises at least 9 mammalian chloride channels. Each is believed to have 12 transmembrane domains and intracellular N and C termini. Mutations in CLCNKB result in the autosomal recessive Type III Bartter Syndrome. CLCNKA and CLCNKB are closely related (94% sequence identity), tightly linked (separated by 11 kb of genomic sequence) and are both expressed in mammalian kidney.1) Gorgojo, J.J., et al., (2006) Horm. Res. 65 (2), 62-68. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CLCNKB. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| CLCNKB antibody can be used for detection of CLCNKB by ELISA at 1:312500. CLCNKB antibody can be used for detection of CLCNKB by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CLCNKB |
|---|---|
| Alternative Names | CLCNKB CLCKB ClC-K2 ClC-Kb |
| Molecular Weight(MW) | 76 kDa |
Application
Antibody used in WB on Human kidney at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1305 - Human Kidney Tissue Lysate |
|---|---|
| Application Notes | CLCNKB antibody can be used for detection of CLCNKB by ELISA at 1:312500. CLCNKB antibody can be used for detection of CLCNKB by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store CLCNKB antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
