Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.Application
Antibody used in IHC on Human Liver.| Product Name | CLDN1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CLDN1, CLD1, SEMP1, ILVASC |
| Product description | Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. This protein, a member of the claudin family, is an integral membrane protein and a component of tight junction strands.1) Coyne, C.B., et al., (2003) Am. J. Physiol. Lung Cell Mol. Physiol. 285 (5), L1166-L1178. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CLDN1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| CLDN1 antibody can be used for detection of CLDN1 by ELISA at 1:312500. CLDN1 antibody can be used for detection of CLDN1 by western blot at 1.0-5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHuman |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CLDN1 |
|---|---|
| Alternative Names | CLDN1 CLD1 SEMP1 ILVASC |
| Molecular Weight(MW) | 23 kDa |
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Application
Antibody used in IHC on Human Liver.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | CLDN1 antibody can be used for detection of CLDN1 by ELISA at 1:312500. CLDN1 antibody can be used for detection of CLDN1 by western blot at 1.0-5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store CLDN1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
