Application
Immunofluorescence of CLDN1 in HepG2 cells with CLDN1 antibody at 20 μg/mL.Application
Immunocytochemistry of CLDN1 in HepG2 cells with CLDN1 antibody at 5 μg/mL.Application
Western blot analysis of CLDN1 in HepG2 cell lysate with CLDN1 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | CLDN1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CLDN1 Antibody: CLD1, SEMP1, ILVASC, CLD1, UNQ481/PRO944, Claudin-1, Senescence-associated epithelial membrane protein |
| Product description | CLDN1 Antibody: Claudin1 (CLDN1), a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Tight junctions are specialized regions of cell to cell contact consisting of networking strands that act as a molecular gasket for preventing the leakage of ions, water, etc., between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different Claudin composition and CLDN1 expression is often cell type and tissue dependent. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome. CLDN1 and CLDN2 were found to be overexpressed in colonal cancer tissues and may be useful as tumor markers and targets for the treatment of colorectal cancer. Characterization of Claudins expression in human tumors can be an additional diagnostic tool. Recent studies show that CLDN1 has gastric tumor suppressive activity and is a direct transcriptional target of RUNX3. Along with SCARB1, LDL-R, and the tetraspanin superfamily member CD81, CLDN1 has been reported to be an entry factor for the Hepatitis C virus.1) Tepass U. Claudin complexities at the apical junctional complex. Nat. Cell Biol.2003; 5:595-7. |
| Immunogen | CLDN1 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of human CLDN1. The immunogen is located within the last 50 amino acids of CLDN1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| CLDN1 antibody can be used for detection of CLDN1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CLDN1 |
|---|---|
| Alternative Names | CLDN1 Antibody: CLD1 SEMP1 ILVASC CLD1 UNQ481/PRO944 Claudin-1 Senescence-associated epithelial membrane protein |
| Sequence Similarities | 2) Cat. No. 17-011 - Hep G2 Cell Slide |
Application
Immunofluorescence of CLDN1 in HepG2 cells with CLDN1 antibody at 20 μg/mL.
Application
Immunocytochemistry of CLDN1 in HepG2 cells with CLDN1 antibody at 5 μg/mL.
Application
Western blot analysis of CLDN1 in HepG2 cell lysate with CLDN1 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | CLDN1 antibody can be used for detection of CLDN1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CLDN1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CLDN1 Antibody is supplied in PBS containing 0.02% sodium azide. |
