Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet.1) Gonzalez-Mariscal, L., et al., (2003) Prog. Biophys. Mol. Biol. 81 (1), 16072.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CLDN15.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
CLDN15 antibody can be used for detection of CLDN15 by ELISA at 1:12500. CLDN15 antibody can be used for detection of CLDN15 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
CLDN15 antibody can be used for detection of CLDN15 by ELISA at 1:12500. CLDN15 antibody can be used for detection of CLDN15 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store CLDN15 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt