Application
immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western Blot at 1:1000 dilution + human heart lysate Lysates/proteins at 20 ug per lane.Application
Western blot analysis of CLDN2 using rabbit polyclonal CLDN2 Antibody (Y224) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CLDN2 gene.| Product Name | CLDN2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Claudin-2, SP82, CLDN2 |
| Product description | This gene product belongs to the claudin protein family whose members have been identified as major integral membrane proteins localized exclusively at tight junctions. Claudins are expressed in an organ-specific manner and regulate tissue-specific physiologic properties of tight junctions. This protein is expressed in the intestine. Alternatively spliced transcript variants with different 5' untranslated region have been found for this gene.1) Smith, A.J., et al. J. Acquir. Immune Defic. Syndr. 55(3):306-315(2010) |
| Immunogen | This CLDN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-227 amino acids from the C-terminal region of human CLDN2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CLDN2 |
|---|---|
| Alternative Names | Claudin-2 SP82 CLDN2 |
| Molecular Weight(MW) | 25 kDa |
Application
immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western Blot at 1:1000 dilution + human heart lysate Lysates/proteins at 20 ug per lane.
Application
Western blot analysis of CLDN2 using rabbit polyclonal CLDN2 Antibody (Y224) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CLDN2 gene.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
