Application
Western blot analysis of CNOT4 in A549 lysate with CNOT4 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | CNOT4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CNOT4 Antibody: NOT4, NOT4H, CLONE243, NOT4, CCR4-NOT transcription complex subunit 4, CCR4-associated factor 4 |
| Product description | CNOT4 Antibody: CNOT4 is a component of the CCR4-NOT transcription complex, a complex that is implicated in the repression of RNA polymerase II transcription. In the CCR4-NOT complex, CNOT4 acts as an E3 ubiquitin-protein ligase and interacts with a subset of E2 ubiquitin-conjugating enzymes through a unique C4C4 RING domain. This E3 ligase activity was shown to be dependent on the selective and specific interaction with the ubiquitin conjugating enzyme UbcH5B. In yeast, mutations in CNOT4 that prevented its interaction with the UbcH5B homolog UBC4 caused increased sensitivity to hydroxyurea, heat shock, and hygromycin B, suggesting that CNOT4 and UbcH5B are involved in stress response in vivo. Multiple isoforms of CNOT4 are known to exist.1) Albert TK, Hanzawa H, Legtenberg IA, et al. Identification of a ubiquitin-protein ligase subunit within the CCR4-NOT transcription repressor complex. EMBO J.2002; 21:355-64. |
| Immunogen | CNOT4 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of the human CNOT4. The immunogen is located within amino acids 70 - 120 of CNOT4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| CNOT4 antibody can be used for detection of CNOT4 by Western blot at 1 - 2 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CNOT4 |
|---|---|
| Alternative Names | CNOT4 Antibody: NOT4 NOT4H CLONE243 NOT4 CCR4-NOT transcription complex subunit 4 CCR4-associated factor 4 |
Application
Western blot analysis of CNOT4 in A549 lysate with CNOT4 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1203 - A549 Cell Lysate |
|---|---|
| Application Notes | CNOT4 antibody can be used for detection of CNOT4 by Western blot at 1 - 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CNOT4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CNOT4 Antibody is supplied in PBS containing 0.02% sodium azide. |
