Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cofilin (Ab-3).Application
Western blot analysis of lysed extracts from MDA cells using cofilin (Ab-3).| Product Name | cofilin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CFL, HEL-S-15, MADH2, MADR2, CFL, CFL1, COF1, Cofilin |
| Product description | Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.1) Kobayashi M, et al. (2006) EMBO J 25 (4): 713-26. Wang Y, et al. (2005) Biol Chem 280 (13): 12683-9. Smith-Beckerman DM, et al. (2005) Mol Cell Proteomics: 156-68. |
| Immunogen | cofilin (Ab-3) antibody was raised against a peptide sequence around aa. 1~5 (M-A-S-G-V) derived from Human cofilin. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CFL1 |
|---|---|
| Alternative Names | CFL HEL-S-15 MADH2 MADR2 CFL CFL1 COF1 Cofilin |
| Molecular Weight(MW) | 19 kDa |
| Tissue Specificity | This antibody detects endogenous level of total cofilin protein. |
| Entrez Gene | 1072 |
|---|---|
| Protein Accession | NP_000213.1 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cofilin (Ab-3).
Application
Western blot analysis of lysed extracts from MDA cells using cofilin (Ab-3).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
