Application
Western Blot at 1:2000 dilution Lane 1: A2058 whole cell lysate Lane 2: A375 whole cell lysate Lane 3: HL-60 whole cell lysate Lane 4: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Overlay histogram showing A431 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit Ig| Product Name | COL17A1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Collagen alpha-1(XVII) chain, 180 kDa bullous pemphigoid antigen 2, Bullous pemphigoid antigen 2, 120 kDa linear IgA disease antigen, 120 kDa linear IgA dermatosis antigen, Linear IgA disease antigen 1, LAD-1, 97 kDa linear IgA disease antigen, 97 kDa line |
| Product description | COL17A1 encodes the alpha chain of type XVII collagen. Unlike most collagens, collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes, multiprotein complexes at the dermal-epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane.1) Di Zenzo,G., et.al., J. Invest. Dermatol. 130 (4), 1040-1047 (2010) |
| Immunogen | This COL17A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 475-504 amino acids from the Central region of human COL17A1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For FACS starting dilution is: 1:25 For WB starting dilution is: 1:2000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | COL17A1 |
|---|---|
| Alternative Names | Collagen alpha-1(XVII) chain 180 kDa bullous pemphigoid antigen 2 Bullous pemphigoid antigen 2 120 kDa linear IgA disease antigen 120 kDa linear IgA dermatosis antigen Linear IgA disease antigen 1 LAD-1 97 kDa linear IgA disease antigen 97 kDa line |
| Molecular Weight(MW) | 150 kDa |
Application
Western Blot at 1:2000 dilution Lane 1: A2058 whole cell lysate Lane 2: A375 whole cell lysate Lane 3: HL-60 whole cell lysate Lane 4: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Overlay histogram showing A431 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit Ig| Application Notes | For FACS starting dilution is: 1:25 For WB starting dilution is: 1:2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
