Application
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of COL18A1 Antibody in mouse lung tissue lysates (35ug/lane)| Product Name | COL18A1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Collagen alpha-1(XVIII) chain, Endostatin, COL18A1 |
| Product description | COL18A1 is encoding the alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains. The proteolytically produced C-terminal fragment of type XVIII collagen is endostatin, a potent antiangiogenic protein.1) Fu,Y.,et.al., Biochemistry 48 (49), 11655-11663 (2009) |
| Immunogen | This COL18A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 237-263 amino acids from the N-terminal region of human COL18A1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | COL18A1 |
|---|---|
| Alternative Names | Collagen alpha-1(XVIII) chain Endostatin COL18A1 |
| Molecular Weight(MW) | 178 kDa |
Application
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of COL18A1 Antibody in mouse lung tissue lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
