Application
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HL-60 whole cell lysate Lane 3: HT-29 whole cell lysate Lane 4: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | COPS7B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | COP9 signalosome complex subunit 7b, SGN7b, Signalosome subunit 7b, JAB1-containing signalosome subunit 7b, COPS7B, CSN7B |
| Product description | Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, JUN, I-kappa-B-alpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.1) Ota T.,et al.Nat. Genet. 36:40-45(2004). |
| Immunogen | This COPS7B antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 61-95 amino acids from human COPS7B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:2000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | COPS7B |
|---|---|
| Alternative Names | COP9 signalosome complex subunit 7b SGN7b Signalosome subunit 7b JAB1-containing signalosome subunit 7b COPS7B CSN7B |
| Molecular Weight(MW) | 30 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HL-60 whole cell lysate Lane 3: HT-29 whole cell lysate Lane 4: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
