Application
Western blot analysis in L929 cell line lysates (35ug/lane).Application
Western blot analysis in mouse brain tissue lysates (35ug/lane).| Product Name | COPZ1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Coatomer subunit zeta-1, Zeta-1-coat protein, Zeta-1 COP, COPZ1, COPZ |
| Product description | The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity). The zeta subunit may be involved in regulating the coat assembly and, hence, the rate of biosynthetic protein transport due to its association-dissociation properties with the coatomer complex.1) Matsuoka, S., et al. Science 316(5828):1160-1166(2007) |
| Immunogen | This COPZ1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-53 amino acids from the N-terminal region of human COPZ1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | COPZ1 |
|---|---|
| Alternative Names | Coatomer subunit zeta-1 Zeta-1-coat protein Zeta-1 COP COPZ1 COPZ |
| Molecular Weight(MW) | 20 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis in L929 cell line lysates (35ug/lane).
Application
Western blot analysis in mouse brain tissue lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
