Application
Antibody used in WB on Human HepG2 cells at 1.25 ug/ml.Application
Antibody used in WB on Human, Mouse, Rat 1:1000.Application
Antibody used in WB on Human cell lines at: 1:1000 (Lane 1: 50ug HeLa lysate, Lane 2: 50ug 293T lysate, Lane 3: 50ug K562 lysate, Lane 4: 50ug MDA-MB-231 lysate).Application
Antibody used in IHC on Human Heart.Application
Antibody used in IHC on Human kidney.| Product Name | COX4I1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | COX4I1, COX4, COXIV, MGC72016, COX4-1 |
| Product description | Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. COX4I1 is the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme.Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it.1) Williams, S.L., (2004) J. Biol. Chem. 279 (9), 7462-7469. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human COX4I1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| COX4I1 antibody can be used for detection of COX4I1 by ELISA at 1:312500. COX4I1 antibody can be used for detection of COX4I1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | COX4I1 |
|---|---|
| Alternative Names | COX4I1 COX4 COXIV MGC72016 COX4-1 |
| Molecular Weight(MW) | 19 kDa |
Application
Antibody used in WB on Human HepG2 cells at 1.25 ug/ml.
Application
Antibody used in WB on Human, Mouse, Rat 1:1000.
Application
Antibody used in WB on Human cell lines at: 1:1000 (Lane 1: 50ug HeLa lysate, Lane 2: 50ug 293T lysate, Lane 3: 50ug K562 lysate, Lane 4: 50ug MDA-MB-231 lysate).
Application
Antibody used in IHC on Human Heart.
Application
Antibody used in IHC on Human kidney.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | COX4I1 antibody can be used for detection of COX4I1 by ELISA at 1:312500. COX4I1 antibody can be used for detection of COX4I1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store COX4I1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
