Application
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: A549 whole cell lysate Lane 4: U-251 MG whole cell lysate Lane 5: human fetal heart lysate Lane 5: mouse heart lysate Lane 5: rat heart lysate Lysates/proteins at 20 ug per lane.| Product Name | COX5B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cytochrome c oxidase subunit 5B, mitochondrial, Cytochrome c oxidase polypeptide Vb, COX5B |
| Product description | Cytochrome C oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Vb of the human mitochondrial respiratory chain enzyme.1) Yoshida, T., et al. Int. J. Mol. Med. 25(4):649-656(2010) |
| Immunogen | This COX5B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-49 amino acids from the Central region of human COX5B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:2000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | COX5B |
|---|---|
| Alternative Names | Cytochrome c oxidase subunit 5B mitochondrial Cytochrome c oxidase polypeptide Vb COX5B |
| Molecular Weight(MW) | 14 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: A549 whole cell lysate Lane 4: U-251 MG whole cell lysate Lane 5: human fetal heart lysate Lane 5: mouse heart lysate Lane 5: rat heart lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
