Application
Immunohistochemistry of CRBN in rat testis tissue with CRBN antibody at 5 μg/mL.Application
Immunofluorescence of CRBN in rat testis tissue with CRBN antibody at 20 μg/mL.Application
Western blot analysis of CRBN in human testis tissue lysate with CRBN antibody at (A) 0.5 and (B) 1 μg/ml.| Product Name | CRBN Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cereblon, AD-006, DKFZp781K0715, MGC27358, MRT2A |
| Product description | CRBN, a member of the Lon protease protein family, plays a role in brain development (1). It is a component of the DCX (DDB1-CUL4-X-box) E3 protein ligase complex, a complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins and is required for limb outgrowth and expression of the fibroblast growth factor FGF8 (2). CRBN is thought to regulate the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1. It is widely expressed and highly expressed in brain (3,4).1) Xin W, Xiaohua N, Peilin C, et al. Primary function analysis of human mental retardation related gene CRBN. Mol. Biol. Rep. 2008; 35:251-6. |
| Immunogen | CRBN antibody was raised against a 16 amino acid peptide near the carboxy terminus of human CRBN. The immunogen is located within the last 50 amino acids of CRBN. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| CRBN antibody can be used for detection of CRBN by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CRBN |
|---|---|
| Alternative Names | Cereblon AD-006 DKFZp781K0715 MGC27358 MRT2A |
| Molecular Weight(MW) | Predicted: 49 kDa Observed: 54 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (94%) |
| Tissue Specificity | CRBN antibody is human, mouse and rat reactive. At least two isoforms of CRBN are known to exist; this antibody will detect both isoforms. |
Application
Immunohistochemistry of CRBN in rat testis tissue with CRBN antibody at 5 μg/mL.
Application
Immunofluorescence of CRBN in rat testis tissue with CRBN antibody at 20 μg/mL.
Application
Western blot analysis of CRBN in human testis tissue lysate with CRBN antibody at (A) 0.5 and (B) 1 μg/ml.| Positive Control | 1) Cat. No. 1313 - Human Testis Tissue Lysate |
|---|---|
| Application Notes | CRBN antibody can be used for detection of CRBN by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CRBN antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | CRBN antibody is supplied in PBS containing 0.02% sodium azide. |
