Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CREB (Phospho-Ser129).Application
Western blot analysis of lysed extracts from 293 cells untreated (Lane 1) or treated with UV (lane 2) using CREB (Phospho-Ser129).| Product Name | CREB (phospho Ser129) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.1) Chrystelle V. Garat, et al. (2006) Mol. Cell. Biol ; 26: 4934 - 4948. Lilah Rothem, et al. (2004) Mol. Pharmacol ; 66: 1536 - 1543. Darren R. Tyson, et, al. (2002) Endocrinology; 143: 674. Kyung-Woo Park, et al. (2003) Arterioscler. Thromb. Vasc. Biol ; 23: 1364. |
| Immunogen | CREB (Phospho-Ser129) antibody was raised against a peptide sequence around phosphorylation site of serine 129 (I-L-S (p) -R-R) derived from Human CREB. |
| Modification | p-Ser129 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CREB1 |
|---|---|
| Alternative Names | CREB HSP90B HSPC2 HSPCB CREB-1 CREB1 |
| Molecular Weight(MW) | 43 kDa |
| Tissue Specificity | This antibody detects endogenous level of CREB only when phosphorylated at serine 129. |
| Entrez Gene | 1385 |
|---|---|
| Protein Accession | NP_004370.1 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CREB (Phospho-Ser129).
Application
Western blot analysis of lysed extracts from 293 cells untreated (Lane 1) or treated with UV (lane 2) using CREB (Phospho-Ser129).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
