Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CREB (Phospho-Ser133).Application
Western blot analysis of lysed extracts from HeLa cells untreated or treated with UV using CREB (Phospho-Ser133).| Product Name | CREB (phospho Ser133) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CREB1 is a transcription factor that is a member of the bZIP family of DNA binding proteins. It binds as a homodimer to the CRE (cAMP-Responsive Element), an octameric palindrome containing a conserved core sequence, 5-prime-TGACG-3-prime. It consists of two transcript variants encoding respective isoforms produced by alternate splicing. It is mapped to 2q32.3-q34 . It is phosphorylated by several protein kinases and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. CREB1 is crucial for the consolidation of long-term conditioned fear memories, but not for encoding, storage, or retrieval of these memories. It is required for the stability of reactivated or retrieved conditioned fear memories .1) Xing J, et al. (1998) Mol Cell Biol 18 (4): 1946-55. Tan Y, et al. (1996) EMBO J; 15 (17): 4629-42. Hao, M. et al. (1996) J. Biol. Chem. 271, 29380-29385. Mayo LD, et al. (2001) Biol Chem; 276 (27): 25184-9. |
| Immunogen | CREB (Phospho-Ser133) antibody was raised against a peptide sequence around phosphorylation site of serine 133 (R-P-S (p) -Y-R) derived from Human CREB. |
| Modification | p-Ser133 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CREB1 |
|---|---|
| Alternative Names | CREB CCNB CREB-1 CREB1 |
| Molecular Weight(MW) | 43 kDa |
| Tissue Specificity | This antibody detects endogenous level of CREB only when phosphorylated at serine 133. |
| Entrez Gene | 1385 |
|---|---|
| Protein Accession | NP_004370.1 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CREB (Phospho-Ser133).
Application
Western blot analysis of lysed extracts from HeLa cells untreated or treated with UV using CREB (Phospho-Ser133).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
