Creb1 binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE.1) Pittenger, C., et al., (2006) J. Neurosci. 26 (10), 2808-2813.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse CREB1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
CREB1 antibody can be used for detection of CREB1 by ELISA at 1:312500. CREB1 antibody can be used for detection of CREB1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Mouse SP2/0 at 0.2-1 ug/ml.
Positive Control
1) SP2/0 Cell Lysate
Application Notes
CREB1 antibody can be used for detection of CREB1 by ELISA at 1:312500. CREB1 antibody can be used for detection of CREB1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store CREB1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.