Application
Western blot analysis in HT29 cell line lysates (35ug/lane).Application
Fluorescent image of A549 cell stained with CREB1 Antibody .A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CREB1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7unitsApplication
Western Blot at 1:2000 dilution Lane 1: NIH/3T3 whole cell lysate Lane 2: C6 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit Ig| Product Name | CREB1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms.1) Jablonski, K.A., et al. Diabetes 59(10):2672-2681(2010) Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) Chen, G., et al. J. Neurosci. 30(39):13066-13077(2010) Serretti, A., et al.Affect Disord (2010) In pressMelnikova, V.O., et al. PLoS ONE(8), E12452 (2010) : |
| Immunogen | This CREB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 105-132 amino acids from the Central region of human CREB1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFWB |
| For FACS starting dilution is: 1:25 For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CREB1 |
|---|---|
| Alternative Names | Cyclic AMP-responsive element-binding protein 1 CREB-1 cAMP-responsive element-binding protein 1 CREB1 |
| Molecular Weight(MW) | 37 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis in HT29 cell line lysates (35ug/lane).
Application
Fluorescent image of A549 cell stained with CREB1 Antibody .A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CREB1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units
Application
Western Blot at 1:2000 dilution Lane 1: NIH/3T3 whole cell lysate Lane 2: C6 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit Ig| Application Notes | For FACS starting dilution is: 1:25 For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
