Application
Flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of CRHR2 Antibody in NIH-3T3 cell line lysates (35ug/lane)Application
CRHR2 Antibody IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | CRHR2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CRHR2 is a receptor for corticotropin releasing factor.It Shows high-affinity CRF binding. Also binds to urocortin I, II and III. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.1) Yerges,L.M., et.al., J. Bone Miner. Res. (2009) |
| Immunogen | This CRHR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 84-114 amino acids from the N-terminal region of human CRHR2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | CRHR2 |
|---|---|
| Alternative Names | Corticotropin-releasing factor receptor 2 CRF-R-2 CRF-R2 CRFR-2 Corticotropin-releasing hormone receptor 2 CRH-R-2 CRH-R2 CRHR2 CRF2R CRH2R |
| Molecular Weight(MW) | 48 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of CRHR2 Antibody in NIH-3T3 cell line lysates (35ug/lane)
Application
CRHR2 Antibody IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
