Human ESP1/CRP2 protein has two LIM domains, and each shares 35.1% and 77 or 79% identical residues with human cysteine-rich protein (CRP) and rat CRIP, respectively. Northern blot analysis of ESP1/CRP2 in various human tissues showed distinct tissue distributions compared with CRP and CRIP, suggesting that each might serve related but specific roles in tissue organization or function. Using a panel of human-rodent somatic cell hybrids, the ESP1/CRP2 locus was assigned to chromosome 14. Fluorescence in situ hybridization, using cDNA and a genome DNA fragment of the ESP1/CRP2 as probes, confirms this assignment and relegates regional localization to band 14q32.3.1) Tsui, S.K., et al., (1996) Biochem. Mol. Biol. Int. 39 (4), 747-754.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CRIP2.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
CRIP2 antibody can be used for detection of CRIP2 by ELISA at 1:62500. CRIP2 antibody can be used for detection of CRIP2 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Human Placenta at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1309 - Human Placenta Lysate
Application Notes
CRIP2 antibody can be used for detection of CRIP2 by ELISA at 1:62500. CRIP2 antibody can be used for detection of CRIP2 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store CRIP2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.