Fertilization consists of a sequence of specific cell-cell
interactions culminating in the fusion of the sperm and egg plasma
membranes. Recognition, binding, and fusion occur through the
interaction of complementary molecules that are localized to
specific domains of the sperm and egg plasma membranes. In the
sperm, the postacrosomal region or equatorial segment is involved
in sperm-egg plasma membrane fusion. The protein encoded by this
gene is a member of the cysteine-rich secretory protein (CRISP)
family. This protein is expressed in the epididymis, is secreted
into the epididymal lumen, and binds to the postacrosomal region of
the sperm head where it plays a role at fertilization in sperm-egg
fusion through complementary sites localized on the egg surface.
Two isoforms are encoded by transcript variants of this gene.1) Mungall, A.J., et al. Nature 425(6960):805-811(2003)
Immunogen
This CRISP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 162-191 amino acids from the C-terminal region of human CRISP1.
Western blot analysis of lysate from MDA-MB-231 cell line, using CRISP1 Antibody at 1:1000 at each lane.
Application Notes
For WB starting dilution is: 1:1000
Additional Information
Form
Liquid
Storage Instructions
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.