Application
Antibody used in WB on Human K562 cells at 0.2-1 ug/ml.| Product Name | CRKL Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CRKL has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase and plays a role in fibroblast transformation by BCR-ABL. In addition, CRKL has oncogenic potential.1) Zhang, W., et al., (2003) J. Biol. Chem. 278 (26), 23978-23983. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CRKL. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| CRKL antibody can be used for detection of CRKL by ELISA at 1:62500. CRKL antibody can be used for detection of CRKL by western blot at 2 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CRKL |
|---|---|
| Alternative Names | CRKL |
| Molecular Weight(MW) | 34 kDa |
Application
Antibody used in WB on Human K562 cells at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | CRKL antibody can be used for detection of CRKL by ELISA at 1:62500. CRKL antibody can be used for detection of CRKL by western blot at 2 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store CRKL antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
