Application
Immunofluorescence of CRMP1 in mouse brain tissue with CRMP1 antibody at 5 μg/mL.Application
Immunohistochemistry of CRMP-1 in mouse brain tissue with CRMP-1 antibody at 2.5 μg/mL.Application
Western blot analysis of CRMP1 in rat brain tissue lysate with CRMP1 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | CRMP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CRMP1 Antibody: Collapsin-response mediator proteins (CRMPs) are highly expressed in the developing brain where they play major roles in axonal outgrowth, neurite differentiation, and apoptosis. Their continued expression in areas of high synaptic remodeling such as the cerebellum, hippocampus, and the olfactory system suggests that these proteins may also be involved in adult brain plasticity. CRMP-1 was initially identified as a dihydro-pyrimidinase expressed exclusively in brain; later studies have shown that it is involved with neurotrophin (NT) 3-induced neurite formation and outgrowth. CRMP-1 localization switches from axonal to somatodendritic when neurons reach functional maturity, suggesting that it is involved in early neuronal differentiation as well as in later processes related to the survival or death of the newly generated neurons.1) Charrier E, Reibel S, Rogemond V, et al. Collapsin response mediator proteins (CRMPs): involvement in nervous system development and adult neurodegenerative disorders. Mol. Neurobiol.2003; 28:51-64. |
| Immunogen | CRMP1 antibody was raised against a 18 amino acid synthetic peptide from near the amino terminus of human CRMP1. The immunogen is located within amino acids 90 - 140 of CRMP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| CRMP1 antibody can be used for the detection of CRMP1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CRMP1 |
|---|---|
| Alternative Names | CRMP1 Antibody: DRP1 DRP-1 CRMP-1 DPYSL1 ULIP-3 ULIP3 Dihydropyrimidinase-related protein 1 Collapsin response mediator protein 1 |
| Tissue Specificity | This antibody is expected to recognize only the longest isoform of CRMP-1. |
| Entrez Gene | 1400 |
|---|---|
| Protein Accession | NP_001014809 |
Application
Immunofluorescence of CRMP1 in mouse brain tissue with CRMP1 antibody at 5 μg/mL.
Application
Immunohistochemistry of CRMP-1 in mouse brain tissue with CRMP-1 antibody at 2.5 μg/mL.
Application
Western blot analysis of CRMP1 in rat brain tissue lysate with CRMP1 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | CRMP1 antibody can be used for the detection of CRMP1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | CRMP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CRMP1 Antibody is supplied in PBS containing 0.02% sodium azide. |
Data sheet for OM276166