Application
Immunohistochemistry of CRTC2 in human small intestine tissue with CRTC2 antibody at 2.5 μg/ml.Application
Immunofluorescence of CRTC2 in human small intestine tissue with CRTC2 antibody at 20 μg/ml.Application
Western blot analysis of CRTC2 in human small intestine tissue lysate with CRTC2 antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | CRTC2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CRTC2 Antibody: CRTC2, also known as TORC2 (Transducers Of Regulated cAMP Response Element-Binding (CREB)) 2 and the related protein CRTC1 are potent CREB coactivators that are exported from the nucleus in a CRM1-dependent manner via phosphorylation-dependent interactions. Studies suggest that their phosphorylation and nuclear/cytoplasmic shuttling play an important role in the regulation of gluconeogenesis by cAMP. CRTC2 is present in both B and T-lymphocytes and abundantly expressed in the thymus. Its activity is important in regulating the expression of genes involved in cellular energy metabolism while CRTC1 is essential for energy balance and fertility.1) Conkright MD, Canettieri G, Screaton R, et al. TORCs: transducers of regulated CREB activity. Mol. Cell 2003; 12:413-23. |
| Immunogen | CRTC2 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human CRTC2. The immunogen is located within the last 50 amino acids of CRTC2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| CRTC2 antibody can be used for detection of CRTC2 by Western blot at 1 - 2 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CRTC2 |
|---|---|
| Alternative Names | CRTC2 Antibody: TORC2 TORC-2 TORC2 CREB-regulated transcription coactivator 2 Transducer of regulated cAMP response element-binding protein 2 |
| Molecular Weight(MW) | Predicted: 67, 76 kDa Observed: 70 kDa |
| Sequence Similarities | 2) Cat. No. 11-801 - Human Small Intestine Tissue Slide |
| Tissue Specificity | Multiple isoforms of CRTC2 are known to exist. CRTC2 antibody is predicted to not cross-react with CRTC1. |
Application
Immunohistochemistry of CRTC2 in human small intestine tissue with CRTC2 antibody at 2.5 μg/ml.
Application
Immunofluorescence of CRTC2 in human small intestine tissue with CRTC2 antibody at 20 μg/ml.
Application
Western blot analysis of CRTC2 in human small intestine tissue lysate with CRTC2 antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1308 - Human Small Intestine Tissue Lysate |
|---|---|
| Application Notes | CRTC2 antibody can be used for detection of CRTC2 by Western blot at 1 - 2 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | CRTC2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | CRTC2 Antibody is supplied in PBS containing 0.02% sodium azide. |
