Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western blot analysis of hCry2-R579 in mouse liver tissue and HepG2 cell line lysates (35ug/lane)Application
Western blot analysis of lysates from human skeletal muscle and human heart tissue (from left to right), using Cry2 Antibody (R579) at 1:1000 at each lane.Application
Western blot analysis of lysates from human heart, skeletal muscle and liver tissue lysate (from left to right), using Cry2 Antibody(R579) at 1:1000 at each lane.| Product Name | Cry2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Various biochemical, physiological and behavioural processes display circadian rhythms controlled by an internal biological clock. The central ?gears?driving this clock appear to be composed of an autoregulatory transcription/posttranslation-based feedback loop. Cryptochrome 1 (CRY1) and 2 (CRY2) are DNA-binding flavoproteins that bear some homology to blue-light receptors and photolyases. In Drosophila, CRY is a photoreceptor for the circadian clock where it binds to the clock component TIM in a light-dependent fashion and blocks its function. Mammalian CRY1 and CRY2 function via light-independent interactions with circadian genes CLOCK and BMAL1, as well as with PER1, PER2, and TIM. They seem to act as light-independent components of the circadian clock and likely regulate Per1 transcriptional cycling via interactions with both the activator and its feedback inhibitors. Mutant mice not expressing the Cry1 or Cry2 protein display accelerated and delayed periodicity of locomotor activity, respectively. It appears that the combination of both proteins working together is essential to synchronize the organism to circadian phases. A critical balance between Cry1 and Cry2 is required for proper clock function; in complete darkness, double-mutant mice present with instantaneous arrhythmicity, indicating the absence of an internal circadian clock.1) Ozgur, S., et al., Biochemistry 42(10):2926-2932 (2003). |
| Immunogen | This Cry2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 564-593 amino acids from the C-terminal region of human Cry2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | CRY2 |
|---|---|
| Alternative Names | Cryptochrome-2 CRY2 KIAA0658 |
| Molecular Weight(MW) | 67 kDa |
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western blot analysis of hCry2-R579 in mouse liver tissue and HepG2 cell line lysates (35ug/lane)
Application
Western blot analysis of lysates from human skeletal muscle and human heart tissue (from left to right), using Cry2 Antibody (R579) at 1:1000 at each lane.
Application
Western blot analysis of lysates from human heart, skeletal muscle and liver tissue lysate (from left to right), using Cry2 Antibody(R579) at 1:1000 at each lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
