Application
Western blot analysis of anti-CK1a Pab in A375 cell lysateApplication
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CK1a antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | CSNK1A1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Casein kinase I isoform alpha, CKI-alpha, CK1, CSNK1A1 |
| Product description | Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. CK1a can phosphorylate a large number of proteins. This cytoplasmic protein participates in Wnt signaling. It has been demonstrated to phosphorylate CTNNB1 on Ser-45 and to interact with the Axin complex.1) Liu, C., et al., Cell 108(6):837-847 (2002). |
| Immunogen | This CSNK1A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 309-337 amino acids from the C-terminal region of human CSNK1A1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For IHC-P starting dilution is: 1:10~50 For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | CSNK1A1 |
|---|---|
| Alternative Names | Casein kinase I isoform alpha CKI-alpha CK1 CSNK1A1 |
| Molecular Weight(MW) | 39 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Chicken |
Application
Western blot analysis of anti-CK1a Pab in A375 cell lysate
Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CK1a antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For IHC-P starting dilution is: 1:10~50 For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
