Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with CK1g2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western Blot at 1:1000 dilution + HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | CSNK1G2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The casein kinase 1 (CK1) group consists of 12 kinases including CK1, TTBK (tau tubulin kinase), and VRK (vaccinia-related kinase) families. The receptor guanylate cyclase (RGC) group consists of 5 kinases similar in domain sequence to TKs (ANP, CYG).1) Grimwood, J., et al., Nature 428(6982):529-535 (2004). |
| Immunogen | This CSNK1G2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 329-360 amino acids from the C-terminal region of human CSNK1G2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | CSNK1G2 |
|---|---|
| Alternative Names | Casein kinase I isoform gamma-2 CKI-gamma 2 CSNK1G2 CK1G2 |
| Molecular Weight(MW) | 47 kDa |
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with CK1g2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western Blot at 1:1000 dilution + HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
