CSNK2A2 belongs to the protein kinase superfamily, Ser/Thr protein kinase family, CK2 subfamily. It contains 1 protein kinase domain. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. CSNK2A2 participates in Wnt signaling. It phosphorylates 'Ser-392' of p53/TP53 following UV irradiation.1) Gottlieb, D.J., (er) BMC Med. Genet. 8 SUPPL 1, S9 (2007).
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CSNK2A2.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
WB
CSNK2A2 antibody can be used for detection of CSNK2A2 by ELISA at 1:62500. CSNK2A2 antibody can be used for detection of CSNK2A2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
CSNK2A2 antibody can be used for detection of CSNK2A2 by ELISA at 1:62500. CSNK2A2 antibody can be used for detection of CSNK2A2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store CSNK2A2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.