Application
Immunocytochemistry of CTRP2 in Jurkat cells with CTRP2 antibody at 10 μg/mL.Application
Western blot of recombinant CTRP2: (A) 5 ng, (B) 25 ng, and (C) 50 ng with CTRP2 at 1 μg/mL.Application
Immunofluorescence of CTRP2 in Jurkat cells with CTRP2 antibody at 20 μg/mL.| Product Name | CTRP2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CTRP2 Antibody: Adipose tissue of an organism plays a major role in regulating physiologic and pathologic processes such as metabolism and immunity by producing and secreting a variety of bioactive molecules termed adipokines. One highly conserved family of adipokines is adiponectin/ACRP30 and its structural and functional paralogs, the C1q/tumor necrosis factor-alpha-related proteins (CTRPs) 1-7. Unlike adiponectin, which is expressed exclusively by differentiated adipocytes, the CTRPs are are expressed in a wide variety of tissues. These proteins are thought to act mainly on liver and muscle tissue to control glucose and lipid metabolism. An analysis of the crystal structure of adiponectin revealed a structural and evolutionary link between TNF and C1q-containing proteins, suggesting that these proteins arose from a common ancestral innate immunity gene. Of the CTRPs, CTRP2 is most similar structurally and functionally to adiponectin. Recombinant CTRP2 rapidly activated AMPK and MAPK in cultured C2C12 cells, leading to increased glycogen accumulation and fatty acid oxidation.1) Fantuzzi G. Adipose tissue, adipokines, and inflammation. J. Allergy Clin. Immunol. 2005; 115:911-9. |
| Immunogen | CTRP2 antibody was raised against recombinant human CTRP2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| CTRP2 antibody can be used for the detection of CTRP2 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | C1QTNF2 |
|---|---|
| Alternative Names | CTRP2 Antibody: CTRP2 zacrp2 CTRP2 UNQ6349/PRO21054 Complement C1q tumor necrosis factor-related protein 2 |
| Sequence Similarities | 2) Cat. No. 17-005 - Jurkat Cell Slide |
| Tissue Specificity | These proteins are often highly modified post-translationally and migrate in SDS-PAGE at positions other than their predicted size. |
Application
Immunocytochemistry of CTRP2 in Jurkat cells with CTRP2 antibody at 10 μg/mL.
Application
Western blot of recombinant CTRP2: (A) 5 ng, (B) 25 ng, and (C) 50 ng with CTRP2 at 1 μg/mL.
Application
Immunofluorescence of CTRP2 in Jurkat cells with CTRP2 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | CTRP2 antibody can be used for the detection of CTRP2 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | CTRP2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CTRP2 Antibody is supplied in PBS containing 0.02% sodium azide. |
