Application
CTTN Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in Hela cell line lysates (35ug/lane).Application
Western blot analysis of CTTN using rabbit polyclonal CTTN Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CTTN gene.| Product Name | CTTN Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene.1) Croucher, D.R., et al. Mol. Cell. Biol. 30(21):5057-5070(2010) |
| Immunogen | This CTTN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 273-302 amino acids from the Central region of human CTTN. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CTTN |
|---|---|
| Alternative Names | Src substrate cortactin Amplaxin Oncogene EMS1 CTTN EMS1 |
| Molecular Weight(MW) | 62 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Chicken |
Application
CTTN Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in Hela cell line lysates (35ug/lane).
Application
Western blot analysis of CTTN using rabbit polyclonal CTTN Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CTTN gene.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
