Application
Western blot analysis in mouse heart tissue lysates (35ug/lane).Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).| Product Name | CUL2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).1) Park, S.W., et al. APMIS 117(12):880-885(2009) |
| Immunogen | This CUL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 150-179 amino acids from the N-terminal region of human CUL2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CUL2 |
|---|---|
| Alternative Names | Cullin-2 CUL-2 CUL2 |
| Molecular Weight(MW) | 87 kDa |
Application
Western blot analysis in mouse heart tissue lysates (35ug/lane).
Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
