Application
Western blot analysis of CUL4B Antibody Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in Hela cell line lysate.Application
Western Blot at 1:2000 dilution + human testis lysates Lysates/proteins at 20 ug per lane.| Product Name | CUL4B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene is a member of the cullin family. The encoded protein forms a complex that functions as an E3 ubiquitin ligase and catalyzes the polyubiquitination of specific protein substrates in the cell. The protein interacts with a ring finger protein, and is required for the proteolysis of several regulators of DNA replication including chromatin licensing and DNA replication factor 1 and cyclin E. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].1) Aggarwal, P., et al. Cancer Cell 18(4):329-340(2010) |
| Immunogen | This CUL4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-278 amino acids from the Central region of human CUL4B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CUL4B |
|---|---|
| Alternative Names | Cullin-4B CUL-4B CUL4B KIAA0695 |
| Molecular Weight(MW) | 104 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis of CUL4B Antibody Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in Hela cell line lysate.
Application
Western Blot at 1:2000 dilution + human testis lysates Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
